http://rulrepository.ru.ac.bd/handle/123456789/138 Tue, 07 Apr 2026 21:43:36 GMT 2026-04-07T21:43:36Z http://rulrepository.ru.ac.bd/handle/123456789/1127 Study of the Anther Culture and Comparison of GxE Models for Selection of Stable Genotypes in Chilli (Capsicum annuum L.) Islam, Md. Aminul The present investigation consists of the study of anther culture and the study of comparison of GxE models for selection of stable genotypes in chilli (Capsicum annuu,n L. ). The materials were seven chilli varieties, viz., abbreviatum, annumm, acuminatum, nigra, co110ides, cerasiformis and fasciculatum which were tested for ten quantitative characters, such ns NSBMF, NSBFF, PHMF, NPI3FF, NPl3FF, LAMF, LAFF, NLMF, NPBMF and NLFF. Immature anthers of all the seven varieties were used as the main materials in the study of anther culture. MS basal medium supplemented with diITerenl combinations of cytokinins and auxins were used. All the seven varieties produced calli supplemented with 0.1 mg/I NAA + 0.1 mg/I 2-1D + 0.2 mg/I BAP. The range of callus induction was from 1.7 to 6.0%. Three varieties, viz. C. abbreviatum, C. annuum and C. fasciculatum responded well in calli formation in five different media among which abbreviatum was the best. In the study of the comparison of GxE models the range of variation was wide and pronounced for all the characters, indicating that there were genotypic diITerences among the varieties under study. For the analysis of stability, under three models, namely Eberhart and Russell's, Perkins' and Jinks' and Freeman and Perkins' were compared to select the stable genotypes. Following all the three models varieties abbrevialum for PHMF, acuminatum for NPBFF, ahbreviatum, annuum and cerasiformis for PHFF were found to be stable having unit regression co-efficient (bi), non significant deviation from regression ( S2 d,) and high mean perfonnances. Following Eberhart and Russell's model, the linear component in the joint regression analysis was found to be important. In Perkins' and Jinks' model both linear and non-linear components were found to be important. But in Freeman and Perkins' model, only non­linear component was significant. This Thesis is Submitted to the Department of Genetic Engineering & Biotechnology , University of Rajshahi, Rajshahi, Bangladesh for The Degree of Master of Philosophy (MPhil) Tue, 01 Jan 2002 00:00:00 GMT http://rulrepository.ru.ac.bd/handle/123456789/1127 2002-01-01T00:00:00Z http://rulrepository.ru.ac.bd/handle/123456789/972 Genetic control of the Growth of Root Nodules and their Relation with yield in Black Gram (Vigna mungo L. Hepper) Goni, Md. Osman For the ease of study the present work was carried out in three separate sections. Section I: 1t contains the analysis of variance, components of variation, heritability and genetic advance for nine lines in black gram. The twelve quantitative characters such as, date of first flower (DFF), date of maximum flower (DMF), plant height at first flower (PHFF), plant height at maximum flower (PMFF), plant weight at harvest (PWtH), dry plant weight (DPWt), number of false pod per plant (NFPdPP), number of pod per plant (NPdPP), pod weight per plant (PdWtPP), number of seeds per plant (NSPP), seed weight per plant (SWtPP) and 100-seed weight ( I 00-SWt). The collected lines were distinctly different from each other for these characters that justifies their inclusion in the present investigation. The highest phenotypic variation and co-efficient of variability were found for NSPP. The genotypic variation and co-efficient of variability were observed for D50%F and PHFF respectively. The highest heritability and genetic advance as a percentage of mean (GA%) with a value of 42.717 and 16.0939 respectively, were recorded for PHFF. High error component of variation causes a low estimation of heritability. Low heritability as well as low values of genetic advance and genetic advance as a percentage of mean were noted for NSPP. Section II: It deals with the analysis of variance with factorial analysis and correlation co-efficient of root length (RL), shoot length (SL), fresh plant weight (FPWt), fresh root weight (rRWt), root volume (RV), nodule number (NN), nodule weight (NWt), number of pod per plant (NPdPP), fresh pod weight (FPdWt), dry pod weight (DPdWt), number of seeds per plant (NSPP), yield per plant (YPP) and I 00-seed weight ( 100-SWt). Analysis of variance indicated that all lines were significant. Factorial analysis indicated that the individual effect of fertilizers is more important for root and shoot characters than yield, while yield itself showed increase response with combined fertilizer dose instead of single dose particularly nitrogen. However, all the root and shoot characters and yield per ii plant except NPdPP and 100-SWt showed the importance of combined dose ie. NPK in black gram. Correlation study indicated that RL showed positive significant correlation with NPdPP and FPdWt. SL exhibited positive significant correlation with FPWt, FRWt and NN. The correlation of FPWt was found to be positively significant with FR Wt, NN, RV and NWt. FRWt showed positively significant correlation with RV. A significant positive correlation was exhibited by NN with NWt, NPdPP, YPP and 100-SWt. The correlation co-efficient of RV was found to be positively significant with NPdPP and FPdWt. NWt showed positively significant correlation with NPdPP, FPdWt, YPP and 100-SWt and negatively significant correlation with NSPP. Section lll: Factorial analysis and analyses of variance, variability, heritability, genetic advance and genetic advance as a percentage of mean for the six characters like moisture, dry-matter, protein, free sugar, reducing sugar and vitamin C of root nodules were done in this section. In the analysis of variance for the chemical characters of root nodules the lines (L) were found to be significant in different from each other and dose (D) effects were not found at each stage (S), except reducing sugar for dose and free sugar and reducing sugar for stage. Application of the individual dose, N, K, P and combined dose, NP, PK, NK and NPK have no effect in most of the stages for all the characters. Protein showed the highest PCV and GCV among these chemical characters. All the chemical characters, except moisture and dry-matter for root nodules exhibited high heritability and genetic advance as a percentage of mean. ln the calculation of molecular weight of protein and its bands it was found that lines L20, L11, L14 and L 15 contained mostly similar types of protein in seeds and root nodules. While, similarity in regard of molecular weight of protein and its band for nodule and seeds was found in least number of cases in L1R- Regarding the 18 amino acids detected, in maximum two cases all the nine lines were found to be different. However, glycine, methionine, leucine, phenylalanine, lysine and arginine were common for root nodules in all the lines. The concentration of bacterial colonies all the nine lines were different. The highest concentration was found for L20 followed by Lis, L15 and L19. With these results lines L20 and L15 may be considered for further breeding research for high yield in black gram. This Thesis is Submitted to the Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi, Bangladesh for The Degree of Master of Philosophy (MPhil) Tue, 01 Jan 2002 00:00:00 GMT http://rulrepository.ru.ac.bd/handle/123456789/972 2002-01-01T00:00:00Z http://rulrepository.ru.ac.bd/handle/123456789/851 Genetic Impact of The Dwarfing Genes on Some Morpho-Physiological and Anatomical Traits in Bred Wheat Bionomics of Notopterus Notopterus (Pallas, 1769) Jahan, Md. Asraful Protein, when balanced by other foods that supply certain amino acids such as lysine, is an efficient source of protein. Various classes of wheat are used for different purposes. Hard­red wheats of this class are generally used in the manufacture of cakes, biscuits, pastry, and other types of flours. Soft white wheats are used principally for pastry flours and shredded and puffed breakfast foods. In summary, wheat is the major ingredient in most breads, rolls, crackers, cookies, biscuits, cakes, doughnuts, muffins, pancakes, waffles, noodles, pie crusts, ice cream cones, macaroni, spaghetti, puddings, pizza, and many prepared hot and cold breakfast foods. It is also used in baby foods, and is a common thickener in soups, gravies, and sauces. Germ, bran, and malt are additional types of wheat products. Much of the wheat used for livestock and poultry feed is a by-product of the flour milling industry. Wheat straw is used for livestock bedding. The green forage may be grazed by livestock or used as hay or silage. Wheat serves a dual purpose by being grazed in the fall and early spring and then harvested as a grain crop. Industrial uses of wheat grain include starch for paste, alcohol, oil, and gluten. The straw may be used for newsprint, paperboard, and other products. This Thesis is Submitted to the Department of Genetic Engineering & Biotechnology , University of Rajshahi, Rajshahi, Bangladesh for The Degree of Master of Philosophy (MPhil) Fri, 01 Jan 2010 00:00:00 GMT http://rulrepository.ru.ac.bd/handle/123456789/851 2010-01-01T00:00:00Z http://rulrepository.ru.ac.bd/handle/123456789/660 Genetic Divergence, Selection Index and Genotype – Environment Interaction in Lentil (Lens culinaris Medic) Dutta, Amit Kumar The whole work of the present investigation was carried out for the study of variability, selection indices, for identify of stable genotypes and for identification and characterization of genetic diversity of six lentil (Lens culinaris Medic) varieties under three different heads; such as part-I: genetic variability, correlation, path coefficients and selection index; part-II: genotype- environment interaction and part-III: genetic divergence using morphological and biochemical markers. For this, eleven yield and yield contributing characters viz date of first flower (DFF), plant height at first flower (PHFF), number of primary branches at first flower (NPBFF), plant height at maximum flower (PHMF), number of primary branches at maximum flower (NPBMF), number of secondary branches at maximum flower (NSBMF), plant weight per plant (PWPP), number of pods per plant (NPdPP), pod weight per plant (PdWPP), number of seeds per plant (NSPP) and seed weight per plant (SWPP). In part-I, the mean values were highly significant with their respective standard errors and wide range of variation among the means values were obtained, suggesting that characters studied are quantitative in nature and under polygenic control. The highest values of σ2P, σ2G, σ²Y, σ²GY and σE² components of variation and PCV, GCV and G˜YCV were obtained for NSPP. Moderate h2b were obtained for PHMF and high GA values were noted for NSPP, while high GA% value was recorded for PdWPP. In the present investigation, correlation studies showed that genotypic correlation was higher than the respective phenotypic correlation in most of the cases. SWPP showed positive correlation with PWPP, PdWPP and NSPP both at phenotypic and genotypic levels. NPBFF, PHMF and PdWPP had the high direct effect on SWPP both phenotypic and genotypic levels, but NSPP showed high direct effect on SWPP at genotypic level. Considering selection index, the highest expected genetic gain was observed in NPBFF, NPdPP, PdWPP, NSPP and SWPP and with their combination. When the two yield contributing characters viz. PdWPP and SWPP were included in the indices, the expected genetic gain was increased. In part-II, stability performances of different lines were different for different characters. Joint regression analysis revealed that genotype × environment interaction was accounted for by both linear and non-linear functions of environment. . A good number of lines showed stable performances for different characters in different environments. The genotypes which showed stable performances, i.e., adaptable to all environments are Bm1 for NPBFF, Bm2 for PHMF, NSBMF, NPdPP and NSPP, Bm3 for NPBFF, Bm4 for PHMF and PWPP, Bm5 for NPBFF and PdWPP and Bm6 for PWPP and PdWPP. The genotypes which are adaptable to favorable environment are Bm1 for most of the characters except DFF, PHFF and NPBFF, Bm3 for all of the characters except NPBFF and NPBMF, Bm4 for PHFF and NPBMF, Bm5 for NSBMF and Bm6 for all of the characters except PHMF, NSBMF and NPdPP. In part-III, above six lentil varieties with thirteen lines of F2 generations were analyzed through morphological markers and seed storage protein profiling for comparing the genetic divergence. In case of morphological characters, analysis of variance showed significant values for all the characters which indicating that the lines are significantly different from each other. Based on Euclidian distance, Bm1 was noted to be closely related with Bm4 × Bm3 and showed the highest dissimilarity value 0.99. Dendrogram was constructed based on the dissimilarity values of eleven characters and the lines were grouped in two clusters. In this study, seed storage protein profiling showed 70.37% polymorphism among the bands of all lines. The highest polymorphic bands were recorded in Bm5 × Bm3. Six type of seed storage protein, albumin protein was abundant in quantity in all the varieties and as well as all the varieties were polymorphic for lysozyme protein. In this analysis, close relatedness was found among cultivar Bm1 and the crosses Bm3 × Bm4 and Bm6 × Bm1 based on Jaccard’s similarity coefficient and cluster analysis. The highest genetic distance showed between Bm4×Bm1 and Bm5× Bm3 F2 lines. The results of principal component analysis is supported by the results of dendrogram, In this work, results of morphological and biochemical marker analysis suggested that the F2 cross of Bm5Bm3 showed the highest genetic diversity among all the materials investigated and it should better for further breeding work. This thesis is Submitted to the Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi, Bangladesh for The Degree of Master of Philosophy (MPhil) Thu, 01 Jan 2015 00:00:00 GMT http://rulrepository.ru.ac.bd/handle/123456789/660 2015-01-01T00:00:00Z