MPhil Thesis

Factors affecting Dental caries in Rajshahi Region: It's extent, Consequences June 2020 and Prevention

2020-06-01T00:00:00Z

Factors affecting Dental caries in Rajshahi Region: It's extent, Consequences June 2020 and Prevention Parvin, Farhana Dental caries or cavity could also be a senous oral pathological state in most industrial countries, poignant 60-90% of school going kids and additionally the overwhelming majority of adults. Oral health standing of vulnerable people in developing countries tends to be lower priority than various health issues. Consequently few studies square measure done on the oral health standing of the agricultural people in developing countries and explore the possibility indicator of cavity tooth decay but no relationship could also be established between drinkable and caries. Throughout this study it had been aimed to prevent cavity by salt and Ca that's available with drinkable. This study was drained upazilla level in rajshahi region. The dust-covered coliform gift within the drinkable in addition a region of the study. Typically this may be usually a cross sectional descriptive type of study. About 540 respondents of 9 upazillas in Rajshahi region were designated and knowledge was collected by a predesigned datasheet. 60 samples of water were collected from the respondents in every upazilla. The information the knowledge the knowledge concerning caries patients was taken within the data sheet. Sample analysis was wiped out 2 classes. One was chemical analysis and alternative was microbiological analysis of the water. The study represent that the Fluoride content in water that was collected from the respondents of various upazillas expressed in table I to 9. The F worth was found 16.126. The many result was found in Tanore, Puthia, Upazilla. Extremely important was discovered in Bagha, Bagmara, Charghat, Durgapar Upazilla. Whereas non-significant result was found in acid, Godagari, Mohonpur Upazilla. The metallic element content in water expressed in table I 0-18. The, F worth was 41.151. The amount of great was totally different in every upazilla. In Godagari Upazilla the result found is critical. In Paba, Bagha, Charghat, Durgapur, Mohonpur and Tanore the result were extremely important. The Fluoride concentration in water samples collected from totally different upazillas ranges from 0.1231 to 2.3254 ppm. Mohanpur Upazilla contains the best quantity of Fluoride and Godagari Upazilla contains rock bottom quantity of Fluoride. The vary between total of sq. and mean sq. is highest in Mohanpur. The metallic element concentration in water sample collected from totally different upazilla ranges from 27. 94 to 71 gm 1-1. Tanore Upazilla contains the best quantity of metallic element and Charghat Upazilla contains rock bottom quantity of metallic element. The vary between total of sq. and mean sq. is highest in Godagari Upazilla. The microbiological analysis that represent the faecal coliforms contamination of water.------ This Thesis is Submitted to the Institute of Biological Sciences (IBSc) , University of Rajshahi, Rajshahi, Bangladesh for The Degree of Master of Philosophy (MPhil)

Comparative Studies on in Vitro Regeneration, Cell Culture and Artificial Seed Production in Eggplant (Solanum Melongena L.)

2007-01-01T00:00:00Z

Comparative Studies on in Vitro Regeneration, Cell Culture and Artificial Seed Production in Eggplant (Solanum Melongena L.) Rahman, Md. Motiur Protocol establishment for cell culture and artificial seed production for improvement of agricultural crops is a new venture in Bangladesh. Both the research avenues are equally important to afford opportunities for the application of advance biotechnological tools. Towards the improvement of eggplant in Bangladesh, meristem culture, somatic embryogenesis and artificial seed production were the prime objectives of the present investigation. Widely cultivated two local cultivars namely cv. China and cv. Loda were taken for the present study. Different medium compositions were tested for sorting out efficient combinations for the better establishment of apical meristem culture, vital footstep towards developing virus-free cultivars. Shoot tips of 20-25 days old in vitro grown plants of the two cultivars were used for meristem isolation. For primary establishment of isolated meristems, 2.0 mgr1 BAP in MS liquid medium was found to be the most effective. MS semisolid medium having 0.50 mgr1 GA3 alone was found to be the best and 0.50 mgr1 GA3 + 2.0 mgr1 BAP appeared moderate formulation for shoot elongation from the primary established meristem. MS semisolid medium fortified with 3.0 mgr1 IBA was found to be the most effective for root induction. Embryogenic callus induction is the precondition for successful accomplishment of embryogenesis and cotyledon and midrib of the two cultivars were used for this purpose. Auxin-cytokinin combinations were proved more potent for embryogenic callus induction as well as callus growth than that of auxin tried singly in the media. MS medium supplemented with 2.0 mgr1 NM + 0.05 mgr1 BAP was proved to be the best media formulation for induction of embryonic cam in eggplant, when cotyledon showed 85.43% and 82.50% and midrib showed 83.30% and 84.50%, respectively for cv. China and cv. Loda. The embryonic Calli were harvested from the experiment and cultured in agarified MS media supplemented with different hormonal treatments to test their regeneration potentialities. Among the different treatments, 2.0 mgr1 Zeatin + 1.0 mgr1 BAP performed the best for regeneration. In this case, cotyledon showed highest regeneration frequency 87.33% and 86.50%, whereas midrib showed 82.15% and 79.75%, respectively for cv. China and cv. Loda. This Thesis is Submitted to the Institute of Biological Sciences (IBSc), University of Rajshahi, Rajshahi, Bangladesh for The Degree of Master of Philosophy (MPhil)

Mass Propagation and Conservation of Three Important Medicinal Plants: Aloe Vera L., Acorus Calamus L. and Stevia Rebaudiana Bertoni

2007-01-01T00:00:00Z

Mass Propagation and Conservation of Three Important Medicinal Plants: Aloe Vera L., Acorus Calamus L. and Stevia Rebaudiana Bertoni Ahmad, Shahrear The peasant investigation was undertaken to standardize in vitro culture techniques for mass propagation and conservation of two threatened important plant species, Aloe vera L. and Acorus calamus L. of Bangladesh and one n: canty introduced non-sugar natural herbal sweetener, Stevia Reba Diana Bertoni. In case of Aloe vera through proper hormonal treatments it was possible to differentiate direct multiple shoots from shoot tip explants. The best multiple shoot production was achieved in MS+2.0 mg/I BA + 0.5 mg/I Kn + 0.2 mg/I NAA. The optimum pH level of the medium was found to be 6.0. As a carbon source, 13DH was the best and local market 13angladesh sugar at 30-40 g/1 was also found to give the satisfactory result. Agar at a level of 7 g/1 irrespective of brands was highly effective to solidly media. For adventitious rooting of regenerated shoots MMS1 + 0.2 mg/I NAA was proved to be the most favorable. The regenerated plants under ex vitro condition showed normal mode of development and 70% survivalist was achieved when they were transferred to garden soil + compost potting……… This Thesis is Submitted to the Institute of Biological Sciences (IBSc), University of Rajshahi, Rajshahi, Bangladesh for The Degree of Master of Philosophy (MPhil)

Biology of the Parasitoids and Predators of Tribolium spp. (Coleoptera: Tenebrionidae)

2004-01-01T00:00:00Z

Biology of the Parasitoids and Predators of Tribolium spp. (Coleoptera: Tenebrionidae) Rahman, Farhana Two parasitoids and one predator were recovered from the red llour beetle, Tribolium castaneum (Herbst) and the confused flour beetle, T. confi,sum Duval infested wheal flours. lbe larval-pupal parasitoids are Rhahdepyris zeae Waterston and !Iolepyris sylvanidis Brethes (Hymenoptera: Bethylidae) and the predator is Xylocoris jlavipes (Reuter) (Hemiptera: Anthocoridae). Some ac;pects of biology of these parasitoids and.predator were investigated in the laboratory condition on the above two species of Tribolium. R. zeae although a larval-pupal ectoparasitoid showed a decided preference towards the fourth instar larvae and early pupae of the host. The developmental time varied in the male and female adult parasitoids. The male and female of R. zeae completed <.lt:velopmcnt within 19.06:1:0.59 um.I 20.14±0.58 days in 'I: castmwum while in T. confu.mm it was 20.10±0.77 and 21 .20±0.92 days, respectively al 30 ± I °C and 70% R. H. The males are polygamous but the females arc always monogamous. Both the sexes of R. zeae lived maximum days when host was supplied continuously. The female deposited maximum number of eggs when hosts were abundant. The sex ratio of R. zeae always showed female biased and dependent on the size of the host. Ji sylvanidis is also a primary larval-pupal ecloparasitoid of T. castaneum and T. confusum. After emergence the male took the initiative in mating and courtship. The fourth instar larvae were preferred for oviposition. The male of fl. sylva11idis completed life cycle within I 5.50±0.62 and 16.00±0.47 days in T. castaneum and T. confusum whereas the female required I 6.66t:0.72 and 17.15±0.80 days. Females are monandrous while males are polygynous. The hosts fluids were necessary for greater longevity in both the sexes. H. ,\ylvanidi.\· is arrhenolokous. Xylocoris jlavipes (Reuter) is a predator of different larval and pupal stages of Tribolium. The early stage was most preferred than that later stages. The male of X flavipcs completed life cycle within 16.99±1.86 and 17.80±1.30 days in 7'. castancum and T. confusum whereas the female required 16.00± 1.63 and 17.00±1.63 days. The female biased sex ratio of X jlavipes was noticed.----- This Thesis is Submitted to the Institute of Biological Sciences (IBSc) , University of Rajshahi, Rajshahi, Bangladesh for The Degree of Master of Philosophy (MPhil)