http://rulrepository.ru.ac.bd/handle/123456789/137 2026-04-07T21:45:08Z 2026-04-07T21:45:08Z Sikdar, Biswanath http://rulrepository.ru.ac.bd/handle/123456789/1150 2025-07-28T10:25:25Z 2004-08-01T00:00:00Z

Improvement of bitter gourd (momordica charantia l.) Through breeding and biotechnology Sikdar, Biswanath A field experiment was carried out in the Fruit Research Station, Bindpur, Rajshahi during 2001-2003 with 6-pasestal and 5-parental diallel sets to determine the gone effects for different quantitative traits te, vegetative-reproductive and al traits in bitter gourd (Momordica charanis L.) Significant differences were observed for most of the traits. The range of variation was enough for all the traits studied, which showed greater scope for their selection as breeding materials. Varians analysis of diallel exhibited the presence of additive gene effects for most of the traits, except BBB, FBN, FEN, and ETS, PTS, GTT-LP, respectively for vegetative-reproductive and anatomical traits of 5-puntal diallel. Out of eighty-nine traits (in both dillelsets), the item was significant for 83 traits, opt ID, FPL, OD and FV of 6-pereal diallel and ID and FBN of 5-parental diallel. In this investigation, relatively lager value of 'a' component in comparison to those of '&' for all the traits were observed. So, the additive component of variation was more important for all the traits in their inheritance. The additive component 'D' was highly significant for all the traits, except LB12 and DRMF of 6-gatal diallel. Whereas, in 5-perectal dialled for vegetative- reproductive traits out of 35 traits the significant 'D' value was observed only for five traits (e, I, OL, FL, FD and FP. In anatomical traits the significant 'D' was observed in seven traits only. The item H1 was significant for most of the traits. The item 'D' was slightly more predominant in most of the traits than dominant component H. The components H, and F were significant for most of the traits and the values were also higher than their "D" values, which showed the importance of non-additive inheritance. It was observed that the H1 values were smaller than H, in most of the traits. So, equal frequency was present in the parents. The positive sign of Fin most of the traits indicated the presence of dominant genes, but in most of the traits it was non-significant, which indicated that dominant properties of genes was not valid. For most of the traits, was non-significant, epistis was not present in the pants. In 6-parental diallel partial dominance was present in 19 out of 30 traits. Whereas, most of the traits (24) showed over dominance in 5-parental diallel. Most of the traits indicated asymmetry of distribution with+,- alleles. In this experiment most of the traits exhibited excess dominant alleles Le, dominant alleles were more frequent than recessive alleles in punts in both of the diallel se The presence of variability and non additive nature was noted for meat of the tits. The heritability in narrow sense ranged from 15.52 (LBL2) to 99.25% (FV) in 6- Parental diallel, 36.41 (DRB) to 69.35 (FV) and 1.40 (PTS) to 50.15% (OPT-IVB) respectively in vegetative-reproductive traits and anatomical traits in 5-allel The regression of Wr on Fr were unity in most of the traits and therefore, the additive dominance model was satisfactory. Non-allelic interaction was not observed in the parents Combining ability was observed in 39 traits in both the diallel sets, GCA effects of parents and SCA effects in direct and reciprocal crosses were significant with a higher magnitude of C of GCA variance indicating that inheritance of these traits were predominantly governed by additive and additive x additive epistatic components. Among 6-paremal diallel of model-l method-ll, the genotype BGGBI was found to be the best general combiner for the important traits I, DRFF, FL, FV, FW and FFW. The genotypes BGGB4, BGGB13 and BGGB14 were the best the general combiner for DRMF, FNFW and HF/W respectively. Similarly 6-perectal diallel of model-l method-I showed th BGGB12 was the best general combiner for DRME, DRFF, FV, FW FFW and FNFW. For 5-parental diallel, the best performing GCA effect was in BOGB17 for IL, DRB, DRMP, DRFF, FL, FFT. The important traits FW, FFW, SF and FP had no significant general combiner. The parent BGGB20 was the best combiner for most of the anatomical traits. In the 6-parental dialled of model-l method-1, the best crosses with high SCA effects were BOOB14 x BOGB4 for DRMF BGGB6 x BOGB1 for DRFF, BOGB14 BGGB13 for FL, BGGBS BGGB4 for FD, BGGB14 BGGBI3 for FV, BGGB14x BGGB13 for FW, BOGB14 BOGBI3 for FFW, BOGB12 BOGB6 for HF/W, Similarly for 6-pantal dallel of model-t method-II the SCA effects showed that the best specific combinations were BGGB13 BGGB6 for DRMF, BGGB6 BGGB4 for DRFF, BGGB14 BOOB12 for FL, BOOB13 B0084 for FD, BOGB13 BOGBI for FV, BOGB12 BOGBI for FW, BGGB12 BGGB4 for FFW, BGGB13 x BGGBI for FNFW, and BGGB12 BGGB6 for HF/W For 5-parenta Galle, the significant SCA effects were BGGB18 BGCB16 for DRMF, BOOB19 BGGB17 for DRFF, BOGB19 BOGB17 for FL, BOGB17 BGGB16 for FV, BOGB20BGGB19 for FFT and BGGB18 BOOB16 for SWF. For the traits HL, EL, LPL2, LBL2, LP, LBL, LBB, IL, FFN, OL, OW, SL, FD, FFW, SP and FP had no significant cresses. The best areas was BOOB 20x BOGB17 for most of the anatomical traits A high frequency of sprouting from shoot tips (90%) and nodal explants (93%) and shoot differentiation was observed in the primary culture of Momordica charantia L on MS hal medium supplemented with various concentrations and combinations of benzyl adenine (BAP), a-naphthalene acetic acid (NAA), indole-3-butyric acid (IBA) and gibberellic acid (GA). The best response in terms of single shoot formation was observed on MS medium with 2 mg1 BAP + 0.2 mgl NAA and multiple shoots were obtained by culture of shoot tips and nodal explants on MS with BAP (2 mg/l)+ IBA (0.2 ng)+GAs (0.3 mg). This combination was suitable for regeneration and growth of shoots. About 99% rating was obtained from shoots cultured on MS supplemented with IBA (0.6 mg/l). Pls with well developed roots were transferred to soil with survival frequency of 70% The growth of meristem was observed on semisolid MS media when the culture media were supplemented with 0.05mg/1 KIN +0.1 mg/l GA, After three weeks, meristems were transferred to MS media supplemented with BA, KIN, IBA, NAA and IAA singly or in combination for shoot elongation and root initiation. Among the different treatments, MS + 1.0 mg/1 BA + 0.1 mg IBA + 0.3 mg/1 GA, showed good responses for shoot initiation with elongation. On the other hand good rooting was observed when 0.5 mg IBA and 0.1mg/ NAA were used in MS semisolid medium. Ten works old in vitro plantlets were successfully planted in soll through gradual acclimatization. Immature embryos were excised from seeds of two genotypes (GBBG 6 and GBBG 14) of bitter gourd and cultured in invariably underwent callasing followed by multiple shoot formation, but in some cases single shoot was observed MS medium supplemented with 1 μM BAP + 0.01 M1 NAA was found to be suitable for adventitious multiple shoots and root regeneration. Addition of 40-100 mg/l casein hydrolysate (CH) improved shoot and root regeneration efficiency. For single shoot and root formation, MS medium with 0.3521 M1 GA+ 100 ml CH was very effective Aspangine and L-glutamine had no role in to tissue differentiation of immature embryo culture. High frequency of direct organogenesis from immature leaflet explants has been achieved in biter gourd. Here shoot buds were found to develop from leaflet on MS medium containing 3.0mg/l BAP and 0.5mgl IAA within 7-8 weeks. Proliferation of regenerated shoot buds, formation of shoots and their elongation took place in the same medium. Regenerated shoots formed healthy roots when they were further grown on MS median supplemented with 0.5mg IBA. Mutiple shoots from the immature cotyledenory nodes of two genotypes of bitter gourd were induced in MS media with GA, IAA, IBA, NAA, KIN and BAP. The shoots continued to increase in number for 5-6 weeks. On the medium, BAP 2 mg in combination with 0.1 mgl IAA + 2 mg/l GA, was the most effective medium for adventitious shoot proliferation from immature cotyledonary node. BGGB1 and BOGB14 genotypes produced over 84% and 80% shoots, respectively on this medium in 6 from the time at inoculation of primary explants. BOGBI was found to be better in rape than B00814 in most of the treatments. Somatic embryogenesis and organogenesis with subsequent plantlets regeneration were achieved in callus cultures derived from immature cotyledon of bitter gourd using MS semisolid besal medium supplemented with different concentrations and combinations of cytokinin (BAP and KIN) and on (IAA and IBA). Among the different combinations, the media supplied with BAP and IAA were more effective in the induction and proliferation of callus then KIN+ IBA or KIN+ IAA or BAP+ IBA. The highest numbers of somatic embryos were obtained in media fortified with IAA (1.0 mg/l) and BAP (1.0 mg). BAP (2.5 mg) in combination with 0.2 mg/l IAA was found table for shoot organogenesis. Rapid and reproducible transformation system for bitter gourd using Agro bacterium fciens mediated gene delivery was developed. Inmature cotyledonary node and immature cotyledon from green immature fruits that have been allowed to imbibe were used as explant, and regeneration we achieved vie organogenesis and embryogenesis, respectively, Cotyledonary node and cotyledon were co-cultured in MS media with 2.0 mgl BAP +0.1 mg IAA +2.0 mg/l GA and 2.5 mg BAP 0.2 mgl IAA, respectively. Histochemical GUS assay of the explants, exsand shoots (newly formed) released that they were GUS positive. Of the explants so the studied cotyledon was found to be more susceptible to Agrobactreium (LBA4404, pB1121) and infection moreover, successful regeneration was also observed. It was the first step to produce transgenic bitter gourd using this gene delivery method and it will also provide a basis for further research to generate transgenic bitter gourd plants. This Thesis is Submitted to the Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi, Bangladesh for The Degree of Doctor of Philosophy (PhD)

2004-08-01T00:00:00Z Islam, Md. Rezaul http://rulrepository.ru.ac.bd/handle/123456789/1107 2023-08-29T08:06:19Z 2003-01-01T00:00:00Z

Genetic Variation in Biochemical Traits of Momordica Dioica Islam, Md. Rezaul Among the vegetables, kakrol (teasle gourd) is a dioecious fruit Vegetables grown almost everywhere in Bangladesh and neighbouring countries. It has become an important and probably the most expensive summer vegetable in Bangladesh (Rashid, 1976). It has good transportability and long shelf life.Its centre of origin is unknown. But its wild types are found in the Himalayas, Chittagong Hilltract, Hills of Rajmahal, Hazaribagh, Rajgir in Bihar, hill region of the Rajasthan and SriLanka.The genus Momordica of the family. Urticaceae is distributed all over the tropics and subtropics but the largest concentration or species is in tropical Africa, Asia and South America (Sheshadri, 1986). It is a summer vegetable of lndo-Malayan origin (Rashid 1976, Singh, 1990). It is also grown luxuriantly in hot humid summer with minimum care. There is a good prospect of commercial production of kakrol for its export potential ns well as internal demand. Recently teasle gourd has become a major vegetable in Bangladesh because of its high export potential and demand in internal market. It is primarily exported to United Kingdom and Middle East countries. Its availability during March to October, the lean period of vegetables also acts in favour of its high demand. It may be cooked in different methods, fired or cooked with or without meat or fish and thus gives high palatability. This Thesis is Submitted to the Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi, Bangladesh for The Degree of Doctor of Philosophy (PhD)

2003-01-01T00:00:00Z Roy, Apurba Kumar http://rulrepository.ru.ac.bd/handle/123456789/850 2022-09-13T08:08:08Z 2010-01-01T00:00:00Z

Genetic variability of lablab bean (Lablab purpureus) and its implication in intercropping Roy, Apurba Kumar Bangladesh is an agro based country. Unfortunately the total food production is not enough to meet up the requirement of her population. Since the land area in our country is limited in respect of population. Bangladeshi people not only suffer from food deficient but also suffer from different nutritional deficiency like protein and calories as well as vitamins and minerals. All such malnutrition problems could have been considerably reduced if the people would have adequate access to vegetable of different kinds which are known to be rich in the entire requirement. Legume crop of our country meet up a vital portion of food and nutrition especially protein and carbohydrate. Some vegetables of legumenoceae family are very much popular for its own performance. Lablab bean is one of them. Lablab been has high yielding more adaptive capability in adverse consequence of environment. Many biochemical properties of bean pod is very much essential for fulfilling the requirement of human health. A wide range of variation is seen in lablab bean genotypes which cultivate in Bangladesh. Growing season, flowering pattern, pot shape, size, colour, biochemical composition showed many differences. Due to the circumstances the experimental and analytical apprise is needed to selecting practicable genotypes. In this study as many as thirteen quantitative and qualitative characters were analyzed by standard biomaterial analysis. Genetic variability was done on the field performance at four year of trial in Fruit Research Centre, Saympur, Binodpur, Rajshahi and Botanical Garden, Rajshahi University Campus. The result that the genotypic phenotypic and environmental effect on lablab bean. mean, standard error, variance, covariance, heritability, genetic advance analysis reported that have high genotype variation in al the selected characters for its mean difference, high heritability and genetic advance. Regression analysis in phenotypic and genotypic level showed significant results in many of the characters. The study on genotype and environmental interaction state that all of the yield and yield contributing characters were quantitative in nature and environmental impact is high. But in the nutritional traits only the protein and lipid value of green young pod has a promising breeding value. The climatic conditions of Bangladesh are suitable for cultivation of several vegetables. There is an excellent opportunity for the cultivation of multiple vegetables in one season in our country. For these regards in the experiment some winter vegetable and spices salad crop like onion, letuce, amaranth, puisak, stem amaranth were intercrop with popular main crop lablab bean. The result of the experiment revealed that all of the vegetables were more or less profitable than sole bean cultivation. As the result of first year experiment leafy vegetables were chosen for selecting more suitable intercropping model, lettuce showed highest benefit according to the cost benefit analysis and land equivalent ratio followed by red amarant and amaranth. Intercropping not only provides opportunity of effective utilize of land but also helps in reducing the risk of economic failure in case of natural hazards of market condition. It should determine to increase crop production in this limited land to meet up the food and nutritional deficit in this huge populated small country Bangladesh. From the methodological screening of lablab bean genotypes according to the adaptability high yielding capability and better notional values GBLB6 and GBLB9 are promising for fulfilling our requirement. At the same time some vegetables such as lettuce, amarant, red amaranth can provide substantial yield advantages compared to sole cropping. Wide range of variation is shown in lablab bean genotype especially yield contributing characters such as number of raceme-per plant, number of pod per raceme and also denote the great opportunity in suitable nutritional traits like protein and lipid content. Selecting by averse character study in variability and GxE suitable bean genotypes interaction were appropriate to cultivate with many other companion crop. The recommendation of the project is that most of the people of Bangladesh can achieve adequate food, proper nutrition and more benefit in agricultural practices. This Thesis is Submitted to the Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi, Bangladesh for The Degree of Doctor of Philosophy (PhD)

2010-01-01T00:00:00Z CHOWDHURY, ANURADHA ROY http://rulrepository.ru.ac.bd/handle/123456789/757 2022-08-16T03:47:40Z 2013-01-01T00:00:00Z

Study of Genetic Architecture on yield and Some of the Yield Components in Lentil (Lens Culinaris Medic.) CHOWDHURY, ANURADHA ROY Inheritance of the yield and yield contributing characters of six lines of lentil (Lens culinaris Medic.) was studied in 2005-2009 through diallel, combining ability, heterosis and model fitting in the first part (Part I) consisting of two experiments. Twelve yield contributing characters viz., days to flower (DF), plant height at first flower (PHFF), number of primary branches at first flower (NPBFF), number of secondary branches at first flower (NSBFF), canopy area at maximum flower (CAMF), number of secondary branches at maximum flower (NSBMF), number of pods per plant (NPdPP), pod weight per plant (PdWPP), number of seeds per plant (NSPP), seed weight per plant (SWPP), individual plant weight (IPlW) and root weight (RW) were studied in a six parental half diallel analysis in experiment I. In experiment II, above characters were considered for study of heterosis and model fitting. The combining ability analysis in lentil showed that the variation due to gca was found to be significant for the characters namedly DF, PHFF, CAMF and RW and variance due to sea was non significant for all of the characters. Component variance due to gca (clg) was higher than that of due to sea (cr2s) for DF, NPBFF, CAMF, PdWPP, SWPP and IPlW. Additive genetic component (cr2A) was greater than dominance component (cr2D) for DF, PHFF, NPBFF, CAMF, PdWPP, SWPP, IPIW and RW. From the comparison of gca effects of individual parents for twelve characters, positive significant gca effect was seen for DF by P 4, for PHFF by P2 and P3, for NSBFF by P 4, for CAMF by P2 and P3, for IPlW by P2 and for R W by P2 and P4. The negative and significant gca effect was obtained for DF by P3, for PHFF by P1 and for NPBFF, CAMF, NPdPP, PdWPP, NSPP, SWPP, IPlW and RW by P6 in experiment I. P4 for NSBFF, NPdPP, NSPP and RW, P2 for PHFF, CAMF, PdWPP SWPP and IPlW, P5 for NPBFF and NSBMF and P3 for DF performed as better combiner. P 1 xP2 performed good specific combiner for NSBFF, Pd WPP, SWPP and RW and P1 xP3 for CAMF, NSBMF, NPdPP and IPIW. In the present study, the ratios of [(H1/D)] 112 suggested over dominance for NSBFF, NSPP, SWPP, IPIW and RW, whereas partial dominance was recorded for the remaining characters except NPBFF, NPdPP and PdWPP in F1 generation. In F2 generation over dominance was found for DF, NPBFF, NSBFF, NSBMF, NPdPP, NSPP and SWPP, whereas partial dominance was shown by PHFF, CAMF, IPIW and RW. Only one group of genes controlled the characters namedly DF, NPBFF, NSBFF, CAMF, NSBMF, NPdPP, PdWPP, NSPP, SWPP, IPIW and RW and two group of genes controlled PHFF in F1 generation, whereas in F2 generation one group of genes controlled the characters viz. DF, PHFF, NSBFF; six groups of genes controlled the character NPBFF; four groups of genes controlled the characters viz., CAMF and NSBMF; three groups of genes controlled NPdPP; two groups of genes controlled PdWPP, NSPP and SWPP; ten groups of genes controlled IPlW and seven groups of genes controlled RW. From graphical analysis, it was evident that array 1 possessed dominant gene in excess for PHFF of replication 2, for CAMF of replication 2 and for IPlW of replication 2 in F 1 generation. Array 2 possessed dominant gene in excess for R W of replication 2, for DF and for NSPP of replication total in F 1 generation and this array possessed dominant gene in excess for NPBFF of replication 2, for NSBFF of replication 1, for PdWPP of replication 2, for NPBFF, NPdPP and PdWPP of replication total in F2 generation. Array 3 possessed dominant gene in excess for NSBMF of replication 2 and for NPdPP of replication 2 in FI generation and for NPdPP of replication 1, for SWPP of replication 1 and for SWPP of replication 1 in F2 generation. Array 4 possessed dominant gene in excess for NSPP of replication 1, for PHFF, NSBMF and NPdPP of replication total in FI generation and for NPBFF of replication 1, for CAMF of replication 2 and for IPlW in F2 generation. Array 5 possessed dominant gene in excess for CAMF of replication 1, for NPdPP of replication 1, for IPlW of replication 1, for NPBFF, CAMF and IPlW of replication total in F1 generation and for NSBMF of replication 2, for NSPP of replication 1, for SWPP of replication 2, for IPlW of replication 1, for RW of replication 1, for NSPP, SWPP and RW ofreplication total in F 2 generation. Array 6 possessed dominant gene in excess for PHFF of replication 1, for NPBFF of replication 1, for NSBMF replication 1, for NSPP of replication 2, for PdWPP and SWPP in F1 generation and for PHFF of replication 1, for PHFF of replication 2, for CAMF of replication 1, for PHFF and CAMF of replication total in F2 generation. Array 1 possessed recessive gene in excess for PHFF of replication 1, for CAMF of replication 1, for NSBMF of replication 1, for NPdPP of replication 1, for NPdPP of replication 2, for NSPP of replication 1, for IPl W of replication 1, for PHFF, CAMF, NSBMF, NPdPP, PdWPP, SWPP and IPlW of replication total in F, generation. Array 1 possessed recessive gene in excess for NPBFF of replication 1, for CAMF of replication 2, for Pd WPP ofreplication 2, for NSPP ofreplication 1, for SWPP ofreplication 1, for SWPP ofreplication 2, for NPdPP, PdWPP, NSPP, SWPP and IPlW of replication total in F2 generation. Array 2 possessed recessive gene in excess for NPBFF of replication 1 and for NSPP of replication 2 in FI generation and for PHFF of replication 1, for PHFF of replication 2, for IPlW of replication 1, for PHFF and CAMF of replication total in F2 generation. Array 3 possessed recessive gene in excess for DF and NSPP of replication total in FI generation. This array possessed excess of recessive genes for NPBFF of replication 2, for CAMF of replication 1, for NSBMF of replication 2 and for NPBFF of replication total in F2 generation. Array 4 possessed recessive gene in excess for PHFF of replication 2, for CAMF of replication 2, for IPlW of replication 2 and for RW of replication 2 in F1 generation. This array possessed recessive in excess for NSBFF of replication 1, for RW of replication 1 and for RW of replication total in F2 generation. Array 5 possessed recessive gene in excess for NSBMF of replication 2 in FI generation. Array 6 possessed recessive gene in excess for NPBFF in FI generation and for NPdPP of replication 1 in F2 generation. Array 3 possessed more or less equal proportion of dominant and recessive genes for most of the characters in both generations. In heterosis study, P1 xP2 showed the highest value of mid parent and better parent heterosis for NSBFF, PdWPP, SWPP and RW. From joint scaling test, it was revealed that non significant x2 value was obtained by all of the crosses for SWPP. From the inheritance study through diallel and heterosis, it was found that P1 xP2 and P1 xP3 was the promising crosses in respect of PdWPP, SWPP and RW. These crosses appeared important for heterosis study. In second part (Part-II) of the present investigation, FI materials of half diallel crosses for nine characters viz., days to flower (DF), plant height at first flower (PHFF), number of primary branches at first flower (NPBFF), number of secondary branches at first flower (NSBFF), canopy area at maximum flower (CAMF), pod weight per plant (PdWPP), seed weight per plant (SWPP), individual plant weight (IPlW) and root weight (RW) were studied for correlation, path-coefficient and selection index. Phenotypic component of variation ( cr2p) was higher than genotypic (cr2g) component of variation. The highest genotypic and phenotypic components of variations were obtained for CAMF. In the present materials, high genotypic values caused high phenotypic values. In this investigation, genotypic correlations were higher than the respective phenotypic correlations for most of the characters. SWPP showed highly significant and positive correlation co efficient with other characters except NPBFF at genotypic level and except NPBFF and DF at phenotypic level. The highest significant and positive genotypic correlation coefficient was recorded for NSBFF with PdWPP at genotypic level and PdWPP with SWPP at phenotypic level. PdWPP had the highest positive direct effect on SWPP at both genotypic and phenotypic level. The maximum expected genetic gain of 4603 .196% was found when NPBFF and R W were included in the discriminant function. These two characters had high correlation coefficient with most of the characters studied as well as direct effect at genotypic level may be considered as primary yield components. This Thesis is Submitted to the Department of Genetic Engineering & Biotechnology, University of Rajshahi, Rajshahi, Bangladesh for the degree of Doctor of Philosophy (PhD)

2013-01-01T00:00:00Z